Therapeutic Efficacy of Topical Sesame and Garlic Ointment Extract on Second-Degree Burn Wounds in Rabbits: A Comparative Histopathological Study

Abstract

Background: The public health implications of burn injuries are substantial, and conventional burn treatments can result in prolonged healing time and negative side effects. Recently, there has been increased interest in using natural products and their active ingredients for use as alternative therapies for wound care. The purpose of this investigation was to determine the use of a new topical ointment composed of sesame oil and garlic extracts for treating second-degree burn injuries. Objective: The effects of sesame/garlic ointment extract on wound healing will be tested by measuring the area of the wound, the time required for the epithelial layer to heal, and the effects of the ointment on the appearance of histopathological changes in the tissue of the rabbits. Methods: A total of fifty New Zealand rabbits were randomly separated into two groups (n=25) as follows: Group A (the control group; received standard petroleum jelly) and Group B (the treatment group; received sesame and garlic ointment). Second-degree burn injuries were inflicted to the dorsum (back) of each rabbit. The sesame and garlic ointment was created using cold-pressed sesame oil containing sesamol and an alcoholic garlic extract (15% concentration containing allicin) blended with a base of petroleum jelly. The ointment was applied to the wounds in both groups twice daily for 14 days, and measurements of the area of each wound were recorded on days 0, 7, and 14. Tissue was also collected from the rabbits on days 7 and 14 for histopathological evaluations. Download Full Text