Histochemical study of the Jujenum in two types of bird’s Guinea fowl (Numidia meleagris) and Falcon (F. tinnunculus)

Abstract

Twenty birds were used to conduct the current study, aged 1-2 years, the Jejunum samples were collection from September 2024 to February 2025, the birds collected at Al-Samawa abattoir and the study was conducted in department of anatomy, histology in college of veterinary medicine university of AlMuthanna. The Jejunum wall consists from the four common known layers of a tube organ: tunica mucosa, tunica submucosa, muscularis Externia and tunica serosa and adventitia. Histochemical by applying three stains: PAS, AB(PH-2.5) PAS-AB [pH 1] stain. These staining techniques were conducted to view the presence or absence of neutral mucins, acidic mucins and neutral mucins with acidic mucins respectively. Both two types of cells of villi, which were the simple columnar cells that reacted negatively with PAS technique and the goblet cells which reacted strongly with this technique. By using the alcian-Blue(PH-2.5) stain the goblet cells were revealed the strong reaction in both villi and the crypt of lieberkühn . These reactions of AB stain indicate presence of acidic mucins in the villi and crypts. On using the combined PAS-AB (PH-1) for staining the tissues of Jejunum wall in guinea fowl, the columnar epithelial cells reacted negatively, whereas the goblet cells reacted moderate with this stain, indicating the presence of neutral and acidic muccopolysaccharide, the connective tissue of both lamina propria, submucosa and serosa sustain negative reaction. The smooth muscle fibers of muscularis mucosa and tunica muscularis afforded negative reaction with the PAS stain, AB and PAS + AB. Download Full Text