Anti cancer potential of green biocatalysts (Si@OSA-Dir.Si@SA) using carbonic anhydrase

الملخص

Carbonic anhydrase (CA) is an important biological target in cancer therapy, due to its vital role in regulating intracellular pH and providing an acidic environment that promotes the growth and spread of cancer cells. Tumor cells rely on aerobic glycolysis, which leads to the accumulation of acidic products such as CO₂ and lactic acid, thus lowering the pH in tumor tissue. In this study, molecular docking demonstrated that SA and OSA possess a high binding capacity to the active site of CA II, with SA recording the highest binding energy (-8.13 kcal/mol). Hydrogen bonding was documented within the active pocket, supporting the stability of the enzyme complex. CA was purified from red blood cells using systematic steps that included hypotonic lysis, hemoglobin removal using chloroform and ethanol, and precipitation with ammonium sulfate. Subsequently, the effectiveness of nanocatalysts (Si@SA and Si@OSA-Dir) at different concentrations (up to 500 µg/mL) was evaluated, demonstrating their ability to raise the pH of the medium and reduce acidity. The Si@OSA-Dir catalyst demonstrated significant effectiveness in stabilizing pH at 8 within 20 minutes, while the other compounds reached pH 7.7, demonstrating their effectiveness in adjusting acidity.The results indicate that these prepared compounds hold promise as CA inhibitors and have potential applications in cancer treatment strategies by targeting the acid balance in the tumor microenvironment. Download Full Text