Study of Drug Sensitivity and Some Virulence Factors in Epicoccum nigrum and Bipolars sorokiniana Fungi Isolated from Transport Vehicles
الملخص
A total of 230 samples were collected from various modes of transportation (private and public cars and trains), two isolates of the fungus Epicoccum nigrum and two isolates of the fungus Bipolaris sorokiniana were obtained. These isolates were identified based on the morphological characteristics of the fungal culture as well as microscopic characteristics, and their diagnosis was confirmed molecularly based on the 18SrRNA gene using PCR technology. Some virulence factors (protease enzyme, lipase enzyme, and biofilm) were investigated in two isolates of the fungus Epicoccum nigrum and two isolates of the fungus Bipolaris sorokiniana, The results of the phenotypic detection of the protease enzyme showed that all isolates gave a positive result, i.e., they showed their ability to secrete this enzyme. The gene responsible for the secretion of this enzyme was detected molecularly, and the phenotypic detection of lipase secretion was positive in all isolates studied. that all these isolates contained the lipase secretion gene through molecular detection. The results were negative in the morphological detection of biofilm formation in all isolates. I conducted a sensitivity test on the Epicoccum nigrum and Bipolaris sorokiniana fungal isolates, both morphologically and molecularly, for a number of antifungal agents, namely fluconazole, nystatin, and amphotericin-B. None of the Bipolaris sorokiniana isolates showed resistance to any of these antifungals, while one of the Epicoccum nigrum isolates showed resistance to the antifungals Nystatin and Amphotericin-B, and the second isolate of this fungus showed resistance to Fluconazole. The results of molecular detection of the fluconazole resistance gene showed that one of my Epicoccum nigrum isolates contained this gene, while molecular detection confirmed that neither of my Bipolaris sorokiniana isolates contained the gene under investigation. Download Full Text