Optimization of Catechin-Mediated Inhibition of α-Amylase and αGlucosidase Using Response Surface Methodology: Implications for Glycemic Control.

الملخص

Background: Managing postprandial hyperglycemia is an important target for the treatment of type 2 diabetes mellitus (t2dm). One strategy is to reduce the activity of enzymes responsible for carbohydrate digestion, such as α-amylase and α-glucosidase. In recent years, natural polyphenols like catechin and epicatechin have been studied for their potential metabolic effects. Experimental data suggest potential impacts on glucose metabolism and insulin sensitivity; however, evidence from integrated human models is limited and needs further clarification. Objective: this study examined the effects of catechin and epicatechin supplementation on digestive enzyme activity and glycemic control in individuals with type 2 diabetes, using response surface methodology to analyze metabolic interactions. Methods: in a central composite design study, 192 participants (diabetic and healthy) received 200 mg/day of catechins (100 mg catechin and 100 mg epicatechin twice daily) for 45 days. Fasting serum samples were analyzed for α-amylase, α-glucosidase, and β-glucosidase activities, as well as glucose and insulin levels. Serum underwent dialysis and sequential molecular weight filtration before rp-hplc separation on a c18 column. Enzyme activity was determined spectrophotometrically, and insulin concentrations by elisa. A desirability function, based on the geometric mean of individual outcomes, summarized the overall metabolic response. Anova and goodness-of-fit indicators assessed model performance. Download Full Text